Journal: Frontiers in Veterinary Science
Article Title: Marek's Disease Virus and Reticuloendotheliosis Virus Coinfection Enhances Viral Replication and Alters Cellular Protein Profiles
doi: 10.3389/fvets.2022.854007
Figure Lengend Snippet: Confirmation of four differentially expressed proteins (IRF7, MX1, AKT1, and TIMP3) in MDV and REV coinfected and single infected, and mock-infected CEF cells by Western blotting. β-Actin was used as an internal control to normalize the quantitative data. MDV, Marek's disease virus; REV, reticuloendotheliosis virus; CEF, chicken embryo fibroblast.
Article Snippet: The membranes were incubated at 37°C for 60 min with rabbit polyclonal antibody to TIMP3 (Abcam, Cambridge, UK), rabbit polyclonal antibody to AKT1 (BIOSS, Beijing, China), rabbit polyclonal antibody to MX1 (ProteinTech Group, Chicago, IL, USA), rabbit polyclonal antibody to IRF7 (BIOSS, Beijing, China), mouse monoclonal antibody anti-REV gp90, and rabbit polyclonal anti-MDV pp38.
Techniques: Infection, Western Blot, Control, Virus